The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable DNA polymerases. On 6th of June 2016, novel method to detect  SARS-CoV-2 was introduced introduced in Yasashii news of TV Osaka on June 7, 2021. The method was developed by Dr Itaru Yanagihara, Research Institute, Osaka Women’s and Children’s Hospital, Dr Kiyoshi Yasukawa, Graduate School of Agriculture, Kyoto University and Dr Shinsuke Fujiwara, School of Biological and Environmental Sciences, Kwansei-Gakuin University. In this study, they compared the detection of SARS-CoV-2 by a one-step real-time RT-PCR method using a heat-resistant reverse transcriptase variant MM4 from Moloney murine leukemia virus, two thermostable DNA polymerase variants with reverse transcriptase activity. They blended the enzymes and optimized the reaction conditions to achieve an efficient detection.
(PLoS ONE 16(6): e0252789. https://doi. org/10.1371/journal.pone.0252789)